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This option causes the Cary system to use the deuterium lamp to calibrate the

instrument at 2624.4, 1312.2 and 0.00 nm.

Note

When performing a wavelength calibration you must firstly remove the DRA,

perform the calibration, then return the DRA to the instrument. Refer to the

Wavelength Accuracy

help in the Validate application.

Calibration using Cary Win UV Software

The recommended method for performing calibration is by using the Auto Calibrate

function in the Validate application.

Caution

Both instrument and DRA are calibrated at the factory and should be recalibrated only

when tests indicate the need for it. Failure to complete or the use of incorrect filters

may render the instrument unusable.

Signal processing

This causes the Cary system to calibrate the programmable gain amplifiers on the

photomultiplier tube. This test is performed during initialization of the Cary

4000/5000/6000i instruments.

UV/Vis 0 %T correction

This causes the Cary system to close the shutter and calibrate the UV/Vis detector for

0 %T errors. If a DRA is connected a mask must be placed in front of the transmission

port (rear instrument beam) during the calibration process. The software will prompt

you for this.

Note

This calibration is part of the normal initialization procedure, however

calibration is advised when working at high absorbance where a repeat

calibration after the instrument is fully warmed up would be beneficial.

NIR 0%T correction

This closes the shutter and calibrates the NIR detector for 0 %T errors. If a DRA is

connected a mask must be placed in front of the transmission port (rear instrument

beam) during the calibration process. The software will prompt you for this.

Auto calibrate

Will perform all the calibrations relevant to the instrument and DRA combination

being used. User interaction is required if a DRA is in place.

Wavelength status

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Mantis 5000 Specifiche Pagina 42